沈晴昳,孙皎,刘昌胜,陈芳萍,吴婕.磷酸钙/硅酸钙/泡铋矿复合盖髓材料的细胞毒性和抑菌性评价[J].口腔材料器械杂志,2012,21(1):17-21.
磷酸钙/硅酸钙/泡铋矿复合盖髓材料的细胞毒性和抑菌性评价
Evaluation on cytotoxicity and antimicrobial properties of calcium phosphate/calcium silicate/bismutite cement
投稿时间：2011-08-22  修订日期：2011-09-19
DOI：10.11752/j.kqcl.2012.01.04
中文关键词:  盖髓剂  磷酸钙/硅酸钙/泡铋矿  细胞毒性  抑菌性
英文关键词:Pulp-capping agent  Calcium phosphate/calcium silicate/bismutitc  Cytotoxicity  Antimicrobial
基金项目:上海市重点学科(口腔基础医学)建设项目(No.S30206)
作者单位E-mail
沈晴昳 上海市口腔病防治院, 上海 200031  
孙皎 上海交通大学医学院附属第九人民医院, 上海生物材料研究测试中心, 上海 200011 jiaosun59@yahoo.com 
刘昌胜 华东理工大学材料与工程学院, 上海 200237  
陈芳萍 华东理工大学材料与工程学院, 上海 200237  
吴婕 上海交通大学医学院附属第九人民医院, 上海生物材料研究测试中心, 上海 200011  
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中文摘要:
      目的: 评价新型复合盖髓材料磷酸钙/硅酸钙/泡铋矿复合水门汀(calcium phosphate-calcium silicate-bismuth compound cement,CPCSBi)的细胞毒性和对常她口腔致龋菌的体外抑菌性能。方法: 采用琼脂覆盖法,比较CPCSBi和商品化盖髓材料Dycal、氢氧化钙糊剂(calcium hydroxide,CH)对人牙髓细胞的体外细胞毒性作用。采用琼脂扩散法,通过测量抑菌圈直径大小,评价CPCSBi与Dycal、CH对变形链球菌(Streptococcous mutans,Sm)、粘性放线杆菌(Actinomyces viscosus,Av)、嗜酸乳杆菌(Laetobacillus acidophilus,La)、金黄色葡萄球菌(Staphylococcus aureus,Sa)及白色念珠菌(Candida albicans,Ca)的抑菌效果。结果: CPCSBi表现为轻度细胞毒性,Dycal和CH均显示中度细胞毒性。CPCSBi对Sm、Av、La和Sa具有良好的抑制作用,较Dycal和CH强5～10倍。结论: CPCSBi仅有轻微的细胞毒性,但对口腔常见致龋菌的抑菌作用显著,具有进一步开发的潜能。
英文摘要:
      Objective: The aim of this study was to evaluate cytotoxity and antimicrobal properties of new calciumphosphate/calcium silicate/bismutite(CPCSBi)cement. Methods: Cytotoxity of CPCSBi was compared with those of calcium hydroxide(CH)and Dycal cements in dental pulpcapping applications by an agar overlay test in vitro. The diametersof bacterial inhibition zone of CPCSBi,CH and Dycal against Streptococcous mutans(Sm),Actinomyces viscosus (Av),Lactobacillus acidophilus(La),Staphylococcus aureus(Sa)and Candida albicans(Ca)were determined by an agar diffusion test, respectively. Results: Only slight cytotoxicity appeared for CPCSBi,whereas both CH and Dycal producedmoderate discoloration and lysis. In antimicrobial tests against Sm, Av, Lrr, and Sa, the antimicrobial potency of the CPCS-Bi was approximately 5-10 times greater than that of Dycal and CH groups. Conclusions: These Results suggest that CPCS-Bi possesses slight cytotoxity and high antimicrobial properties.
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