| 沈晴昳,陈芳萍,刘昌胜,孙皎.新型复合盖髓材料对人牙髓细胞分化的体外诱导效果[J].口腔材料器械杂志,2013,22(3):122-125. |
| 新型复合盖髓材料对人牙髓细胞分化的体外诱导效果 |
| Differentiation inducement of calcium phosphate/calcium silicate/bismutite cement to dental pulp cells in vitro |
| 投稿时间：2012-06-04 修订日期：2012-09-21 |
| DOI：10.11752/j.kqcl.2013.03.03 |
| 中文关键词: 盖髓材料 磷酸钙 硅酸钙 泡铋矿 人牙髓细胞 分化 |
| 英文关键词:Pulp-capping agent Calcium phosphate calcium silicate bismutite Hum an dental pulp cell Differentiation |
| 基金项目:上海交通大学医学院博士创新基金(BXJ0731) |
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| 中文摘要: |
| 目的研究新型盖髓材料磷酸钙/硅酸钙/泡铋矿复合水门汀(Calcium phosphate-calcium silicate-bismuth compound cement,CPCSBi)诱导牙髓细胞分化的作用。方法分别采用CPCSBi、氢氧化钙(CH)饱和溶液和10% EDTA溶液,从人牙本质粉末中提取可溶性牙本质基质(DDMCs),通过半定量RT-PCR方法,分析不同材料所提取DDMCs对人牙髓细胞的涎磷蛋白(DSPP)、骨钙素(OCN)、转化生长因子- β1(TGF- β1)基因表达的影响。结果不同材料提取的DDMCs都能提高体外培养人牙髓细胞DSPP、OCN和TGF- β1基因的表达,其中以CPCSBi溶液提取DDMCs的促进作用最明显。结论 CPCSBi对牙髓细胞分化具有促进作用。 |
| 英文摘要: |
| Objective: The aim of this study was to investigate the effects of new calcium phosphate/calcium silicate/bismutite (CPCSBi) cement on differentiation of human dental pulp cells. Methods: Dissoluble dentin m atrix components (DDMCs) were extracted from powdered sound, human dentine samples by either CPCSBi saturated solution, Ca(OH)2 saturated solution, or 10% EDTA, over a 14-day period. The effects of DDMCs extracts on dentin sialophosphoprotein (DSPP), osteocalcin (OCN) and TGF- β1 gene expression in the dental pulp cells were analysed using semi-quantitative RT-PCR following 24 h of exposure. Results: All the DDMCs extracts induced DSPP, OCN and TGF- β1，gene expression in dental pulp cells. While the DDMCs extracted from dentin powder by CPCSBi demonstrated highest inducing effect on expression of DSPP and OCN in human pulp cells. Conclusions: These results suggest that CPCSBi plays an important role in the differentiation of dental pulp cells to odontoblast like cells. |
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