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| 多孔钛的骨传导性及其孔隙结构对MC3T3-E1细胞早期分化的影响 |
| The Osteoconduction and the Influence of Porosity and Pore Size on MC3T3-E1 Differentiation of Porous Titanium |
| 投稿时间：2013-07-20 修订日期：2013-08-01 |
| DOI： |
| 中文关键词: 多孔钛 孔隙率 孔径 细胞迁移 细胞分化 钙结节 |
| 英文关键词:Porous titanium Porosity Pore size Cell migration Cell differentiation calcium nodule |
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| 中文摘要: |
| 【】 目的 探讨多孔钛的骨传导性及其孔隙结构对小鼠前成骨细胞MC3T3-E1早期分化的影响。 方法 碳酸氢铵造孔剂结合粉末冶金法制备6组由不同平均孔隙率范围(A：40.9 %~ 44.3%；B：49.9 %~ 53.3%)及不同平均孔径范围(I：154.8 μm ~ 191.6 μm；II：295.6 μm~ 303.8 μm；III：560.4 μm~ 583.1 μm)组合的多孔钛试样,Ta2级商业致密纯钛作为对照组；每组3个试样。MC3T3-E1细胞接种于24孔板待3 h贴壁后置入多孔钛试样,培养3和5 d后激光扫描共聚焦显微镜(LSCM)观察经FITC微丝蛋白荧光探针标记的细胞。MC3T3-E1细胞接种于已置入试样的24孔板,培养7和14 d后碱性磷酸酶(alkaline phosphatase, ALP)活性检测实验测定细胞的早期分化能力；培养21 d后茜素红染色观察细胞晚期矿化结节。 结果 MC3T3-E1细胞贴壁后,5 d可长入多孔钛孔内及表面。7和14 d多孔钛组ALP活性显著高于对照组(P < 0.05)；其中B-I 组在14 d的ALP活性显著高于其它各组(P < 0.05)。21 d多孔纯钛试样表面及孔隙内均可见钙结节形成。 结论 本实验研究条件下,多孔钛具有一定的骨传导性；平均孔隙率为53.3%且平均孔径为191.6 μm的多孔钛利于MC3T3-E1细胞的早期分化。 |
| 英文摘要: |
| 【】 Objective To Evaluate the osteoconduction and the influence of porosity and pore size on osteoblast differentiation of porous titanium Methods Six groups of porous titanium disk-shape specimens with different porosity ratios (A: 40.9%~ 44.3%；B: 49.9%~ 53.3%) and pore sizes (I: 154.8 μm~ 191.6 μm; II: 295.6 μm~ 303.8 μm; III: 560.4 μm~ 583.1 μm) were fabricated by powder metallurgy process using NH4HCO3 powder as space holder, commercially pure titanium (CpT) Ta2 disk-shape specimens were made as control group. Cell migration ability was measured by putting porous titanium disks into cell culture medium with adhered MC3T3-E1 in it. After 3 and 5days, cell cytoskeleton was stained with fluorescence probe and observed under Laser Scanning Confocal Microscope (LSCM) to observe whether cells migrated onto the disks and into pores. After 7 and 14 days, the ALP activity of MC3T3-E1 seeded on different disks was detected with ALP kit. After 21 days, Alizarin Red S staining test was carried out using a stereomicroscope for extracellular matrix mineralization observation Results After seeding 5 days, the MC3T3-E1 could migrate onto the disks and into pores. After 7 and 14 days, MC3T3-E1 had higher ALP content on porous titanium disks than on CpT disks (P < 0.05). At 14 days, B-I group had secreted more ALP than any other groups (P < 0.05). Alizarin Red S staining results show that calcium nodules have successfully formed both on surfaces and into pores of porous titanium disks. Conclusions The results indicate that MC3T3-E1 can migrate into the pores of porous titanium. Porous titanium with an average pore size of 191.6 μm and a porosity of 53.3% could better promote osteoblast early stage differentiation. |
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