| 李隽,胥春,郝轶,刘晓峰.牵张应变作用下人牙周膜细胞内磷脂酶C-γ1水平变化的实验研究[J].口腔材料器械杂志,2014,23(1):30-34. |
| 牵张应变作用下人牙周膜细胞内磷脂酶C-γ1水平变化的实验研究 |
| Effects of mechanical stretching on PLC-γ1 in human periodontal ligament cells |
| 投稿时间：2013-02-20 修订日期：2013-03-14 |
| DOI：10.11752/j.kqcl.2014.01.07 |
| 中文关键词: 人牙周膜细胞 磷脂酶C-γ1 机械牵张应变 |
| 英文关键词:Human periodontal ligament cells PLC-γ1 Mechanical stretching |
| 基金项目:国家自然科学基金 (31270991)；上海市浦江人才计划资助项目(2013D33) |
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| 中文摘要: |
| 目的 观察人牙周膜细胞(HPDLCs) 内磷脂酶C-γ1 (PLC-γ1) 水平在机械牵张应变作用下的变化。方法 通过原代和传代培养，获得性状稳定的人牙周膜细胞，使用动态机械应变细胞加载仪对细胞进行1%、10% 和20% 动态牵张应变加载。并于加载后10 min、20 min、30 min、40 min、50 min 和60 min，通过流式细胞仪和激光扫描共聚焦显微镜检测细胞内PLC-γ1 水平的变化。应用SPSS13.0 软件包对数据进行统计分析。结果 流式细胞仪检测发现：加载初期，细胞内的PLC-γ1 水平维持在较低水平，随着牵张应变加载时间的延长，牙周膜细胞内PLC-γ1 水平逐渐升高。50 min 时PLC-γ1 水平达到了各个应变组的最高值(P<0.01)，60 min 时各应变组的PLC-γ1 水平都有所下降(P<0.01)。激光扫描共聚焦显微镜检测发现：各组都有PLC-γ1 的表达，其中50 min 加载组表达相对较强。结论 机械牵张应变可以引起人牙周膜细胞内PLC-γ1 表达量发生改变。 |
| 英文摘要: |
| Purpose The purpose of this study is to investigate the effects of mechanical stretching on concentration of PLC-γ1 in the human periodontal ligament cells (HPDLCs). Methods Primany human periodontal ligament cells were obtained and passaged. Then the cells were stretched by dynamic mechanical strain unit with 1%, 10% and 20% strain for different time. PLC-γ1 was examined through Flow cytometry and Laser Scanning Confocal Microscope(LSCM). The data were analyzed using SPSS13.0 software package. Results In early period of stretching, The expression of PLC-γ1 remained at a lower level. Along with the stretching time prolonged, it increased gradually .The level of PLC-γ1 of each stretching group reached peak at 50 minute (P<0.01), then it declined at 60 minute (P<0.01). There was also expression of PLC-γ1 in stretching and control cells by LSCM, but the one in 50 minute stretching cells was more than that of control cells. Conclusion Stretching force induced the change of concentration of the PLC-γ1 of HPDLCs. |
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