| 朱彩莲,李鸣宇,朱来宽,汪俊.根管充填糊剂对根管常见混合细菌的体外抑制作用[J].口腔材料器械杂志,2014,23(2):66-70. |
| 根管充填糊剂对根管常见混合细菌的体外抑制作用 |
| Antibacterial effect of root canal sealers on mixed bacteria in root canals in vitro |
| 投稿时间：2013-07-16 修订日期：2013-12-05 |
| DOI：10.11752/j.kqcl.2014.02.03 |
| 中文关键词: 根管充填糊剂 体外根充模型 混合菌 抑菌 |
| 英文关键词:Root Canal Sealers Root canal-filling model in vitro Mixed bacteria Antibacterial effect |
| 基金项目:国家自然基金(81200774) |
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| 摘要点击次数: 1125 |
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| 中文摘要: |
| 目的 评价3种根管充填材料在体外根充模型中对根管常见混合细菌的抑制作用。方法 收集因正畸治疗而新鲜拔除的下颌双尖牙48颗,常规制备根管,随机分为4组(每组12颗)。实验组分为3组,分别用AH-Plus糊剂、Vitapex糊剂和氧化锌丁香油糊剂加牙胶尖作根管充填,对照组不作充填。根充后行冷热循环处理。使用国际参考菌株变异链球菌、粘性放线菌、嗜酸乳杆菌、粪肠球菌、殊异韦永氏菌、牙龈卟啉菌和核梭杆菌。将等体积混合制备成的混合菌液封入根管。孵育后截取根上1/3、根中1/3、根尖1/3,进行细菌采样、培养,观察细菌菌落数。利用ANOVA(P=0.05)软件,分析各组间的差异。结果 所有的样本均培养出细菌。粪肠球菌在根管的所有部位均有较高的检出率。根管残留细菌总数比较,在根上1/3 Vitapex糊剂抑菌作用优于AH-Plus,而在根尖1/3 AH-Plus糊剂抑菌作用优于Vitapex。结论 根尖1/3处的抑菌作用而言,AH-Plus糊剂优于Vitapex糊剂和传统的氧化锌丁香油糊剂,更具临床意义。 |
| 英文摘要: |
| Objective The objective of the present in vitro study was to investigate the antibacterial effect of three kinds of root canal sealers,AH-Plus,Vitapex and zinc oxide eugenol root canal sealer on mixed bacteria in root canals by a root canal filling model.Methods 48 freshly extracted human mandibular premolars for orthodontic treatment were collected for root canal preparation,and were divided into four groups randomly(n=12).Three experiment groups were filled using the three root canal sealers respectively,leaving a group of blank root canal as negative control,and following by thermal cycling treatment.The international reference strains including Streptococcus mutans,Actinomyces viscosus,Lactobacillus acidophilus,Enterococcus faecalis,Veillonella dispar,Porphyromonas gingivalis and Fusobacterium nucleatum were incubated.Aliquots of mixed bacterial suspension were sealed in the root canal.After incubation,the samples were cross cut to collect bacteria in the apical 1/3,middle 1/3 and coronal 1/3,and then to observe the bacterial colonies.Using the ANOVA(P=0.05)software to analyze the differences between the groups.Results Bacteria were found in all samples.P.gingivalis and F.nucleatum were detected in root canals of the control group,but not in the experiment groups.Lactobacillus and Actinomycetes were missing in the apical 1/3,while the detection rate of E.faecalis was higher in all parts of the canals.Comparing the numbers of residual bacteria in root canal,Vitapex was found to have more effective antimicrobial activity then AH-Plus in coronal 1/3,however,AH-Plus was superior to Vitapex in the apical 1/3.Conclusion Based on the inhibitory effects on bacteria in the apical 1/3 of root canals,AH-Plus has an advantage over Vitapex and conventional zinc oxide eugenol sealer. |
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