| 郭凌云,袁建兵,夏琰,王卓,黄远亮.选择性激光烧结技术构建HA/PCL骨组织工程支架的研究[J].口腔材料器械杂志,2015,24(2):64-68,87. |
| 选择性激光烧结技术构建HA/PCL骨组织工程支架的研究 |
| Study on the fabrication of HA/PCL composite scaffolds using a selective laser sintering technique for bone tissue engineering |
| 投稿时间：2014-10-25 修订日期：2015-02-10 |
| DOI：10.11752/j.kqcl.2015.02.02 |
| 中文关键词: 羟基磷灰石 聚己内酯 选择性激光烧结技术 骨组织工程支架 |
| 英文关键词:Hydroxyapatite Poly-ε-caprolactone Selective laser sintering technique Bone tissue engineering scaffold |
| 基金项目:上海市科委项目(11411963500);上海市浦东新区卫生局优青培养计划(Pw11-3) |
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| 中文摘要: |
| 目的 应用选择性激光烧结技术构建3D 支架, 探讨其作为骨组织工程支架的可行性。方法 以羟基磷灰石(HA)和聚己内酯(PCL)为原材料, 运用选择性激光烧结技术, 制备纯PCL 支架以及HA质量比为5wt.% 和10wt.% 的HA/PCL 支架, 通过扫描电镜观察支架微观形貌, 测定各组支架的孔隙率、抗压强度及亲水性, MTT 法检测10wt.% HA/PCL 支架浸提液的细胞毒性。结果 扫描电镜显示: 支架具有相互连通的三维孔隙结构, 5wt.% 和10wt.% 的HA/PCL 支架孔隙率分别达到78.20% 和80.75%。随着羟基磷灰石含量增高, 抗压强度略有下降, 但支架亲水性提高。MTT 的结果显示: 10wt.% HA/PCL 支架表现出良好的细胞相容性。结论 选择性激光烧结技术制备的HA/PCL 支架具有外形可塑性及良好的孔隙结构, 其力学性能和细胞相容性可支持作为骨组织工程支架应用。 |
| 英文摘要: |
| Objective Novel three-dimensional scaffolds were fabricated using a selective laser sintering technique and investigated for bone tissue engineering applications. Methods The scaffolds were fabricated by hydroxyapatite(HA) (0wt.%, 5wt.% and 10wt.%) /poly-ε-caprolactone (PCL) composite using a selective laser sintering technique. The micro-structural and internal morphologies of the scaffolds were observed by scanning electron microscopy (SEM). The porosities, compressive strength and hydrophilicity of the scaffolds were measured in vitro. Bone marrow stroma cells of dog were cultured in the extracts of the 10wt.% HA/PCL scaffold, and the cytotoxicity was tested by MTT assay. Result In SEM images, the scaffolds showed interconnected pores structure, indicating highly porosities. The porosities of the 5wt.% HA/PCL and 10wt.% HA/PCL scaffolds were respectively 78.20% and 80.75%. With the weight ratios of HA increased, the compressive strength of the scaffolds were slightly reduced but the hydrophilicity of the scaffolds were increased. The MTT data showed that the extract of the scaffolds produced no effect on the cell proliferation and had low cytotoxicity. Conclusions HA/PCL scaffolds fabricated by the selective laser sintering technique has good plasticity and well-defined internal and external structures, indicating that it has high potential as bone tissue engineering scaffolds based on its mechanical properties and biocompatibility. |
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