| 黄争美,马晓飞,Junu Karki,王申春,郎美东,张修银.PEG-CL/LA膜负载甲硝唑药物的体外缓释及抗菌性能研究[J].口腔材料器械杂志,2017,26(2):62-66. |
| PEG-CL/LA膜负载甲硝唑药物的体外缓释及抗菌性能研究 |
| In vitro study of drug release properties and antibacterial activity of medical PEG-CL/LA membranes loaded metronidazole |
| 投稿时间：2016-09-02 修订日期：2016-11-16 |
| DOI：10.11752/j.kqcl.2017.02.02 |
| 中文关键词: 甲硝唑聚乙二醇-己内酯-丙交酯聚合物 药物缓释 抗菌性 |
| 英文关键词:Metronidazole Polyethylene glycol-caprolactone-lactide polymer Sustained-release Antibacterial |
| 基金项目:上海市科委重点基金项目（编号：06JC14048，09JCl408900），上海高校高峰高原学科建设项目 |
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| 中文摘要: |
| 目的 研究以聚乙二醇-聚己内酯-丙交酯（PEG-CL/LA）为聚合物载体的甲硝唑缓释膜的体外抗菌和药物释放性能，旨在研制一种新型的具有抗菌作用的药物缓释膜。方法 用紫外分光光度法测定5wt%、8wt%及10wt%甲硝唑药膜中药物的体外释放度；选用牙周可疑致病菌具核梭杆菌（Fn）和致龋菌变异链球菌（Sm），采用抑菌圈法研究3种药膜对厌氧菌和兼性厌氧菌的抗菌性能。结果（1）药膜在载药量为5wt%~10wt%时具有较好的缓释性能，药物释放时间随着甲硝唑含量的增加而缩短，3种比例的载药膜的药物释放时间均可达7天以上，其中，5wt%药膜可达10天。（2）随着药物含量增加，抗菌作用逐渐增强，5wt%载药膜对Sm和Fn的抑菌圈直径分别为（8.00±0.16） mm和（8.07±0.09） mm；8wt%载药膜为（10.13±0.19） mm和（11.13±0.09） mm；10wt%载药膜为（12.07±0.09） mm和（19.33±0.94） mm。该药膜对具核梭杆菌的抑制作用强于对变异链球菌的抑制作用。结论 PEG-CL/LA载药膜对牙周致病菌的抑制作用效果明显，且具有良好的药物缓释性能，是一种有望用来辅助治疗牙周疾病的药物膜。 |
| 英文摘要: |
| Objective To evaluate drug release properties and antibacterial effect of metronidazole sustained-release membranes made of polyethylene glycol-caprolactone-lactide(PEG-CL-LA)with various drug loadings (5wt%, 8wt% and 10wt%). Methods 1.In vitro drug release properties were examined by ultraviolet spectrophotometry. 2.Antibacterial effect on Streptococcus mutans and Fusobacterium nucleatum was observed on solid culture medium. Results 1. Membranes tested here released drug slowly. Time required for drug release was longer than 7 days, and was decreased by increasing drug loading. 2.Membranes had strong inhibitory effect on both S.mutans and F.nucleatum, and antibacterial activity was enhanced with higher drug loading. Membranes loaded with drug at 10wt% showed larger inhibition zone with a diameter of (12.07 ±0.09) mm and (19.33 ±0.94) mm for S. mutans and F. nucleatum, respectively, (10.13±0.19) mm and (11.13 ±0.09) mm for 8wt%, and (8.00 ±0.16) mm and (8.07 ±0.09) mm for 5wt%. Membranes had greater inhibitory effect on F.nucleatum than on S.mutans. Conclusion Metronidazole sustained-release membranes can potentially be used for the treatment of periodontal disease because of their inhibitory effect on periodontal pathogens and sustained drug-release performance. |
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