| 刘超,孙皎.水解罗非鱼胶原促进人牙周膜细胞活力及成骨分化的效应[J].口腔材料器械杂志,2017,26(2):74-78. |
| 水解罗非鱼胶原促进人牙周膜细胞活力及成骨分化的效应 |
| Hydrolyzed tilapia fish collagen enhances cell viability and osteogenic differentiation of human periodontal ligament cells |
| 投稿时间：2015-06-18 修订日期：2015-10-20 |
| DOI：10.11752/j.kqcl.2017.02.04 |
| 中文关键词: 水解罗非鱼胶原 人牙周膜细胞 细胞活力 成骨分化 整合素-ERK通路 |
| 英文关键词:Hydrolyzed tilapia fish collagen Human periodontal ligament cells Cell viability Osteogenic differentiation Integrin-ERK signal pathway |
| 基金项目:国家自然科学基金项目（编号：31470917）上海市科委项目（编号：15DZ2290100） |
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| 中文摘要: |
| 目的 探讨水解罗非鱼胶原促进人牙周膜细胞活力及成骨分化的作用。方法 制备水解罗非鱼胶原，原代分离培养人牙周膜细胞（hPDL细胞），分别利用MTT试验和real-time PCR试验检测水解罗非鱼胶原对细胞活力和成骨分化相关基因ALP，COL I，OCN和RUNX2的表达的影响，运用激光共聚焦显微镜观察成骨分化相关蛋白骨钙蛋白的表达，进一步采用western blot技术探讨水解罗非鱼胶原对整合素-ERK信号通路的作用。结果 水解罗非鱼胶原能促进hPDL细胞的增殖，使ALP，COL I，OCN和RUNX2的基因上调，诱导骨钙蛋白的产生，这些生物效应与水解罗非鱼胶原可激活整合素-ERK信号通路有关。结论 水解罗非鱼胶原具有骨诱导性，具有诱导hPDL细胞成骨分化的潜能。 |
| 英文摘要: |
| Objective The aim of the present study was to investigate the effect of hydrolyzed fish collagen (HFC) on the viability and osteogenic differentiation of primary human periodontal ligament (hPDL) cells. Methods In this study, the hydrolyzed fish collagen was extracted from tilapia fish scale, and primary hPDL cells were cultured after dissociation. Cell viability was assessed by MTT assay, the expression of osteogenic genes Alp, Col I, Ocn and Runx2 was measured by Real Time-PCR, Osteocalcin protein was detected by immunofluorescent staining, and the role of integrin-ERK signaling pathway in this process was tested by western blotting. Results The results revealed that HFC improved the viability of hPDL cells. The expression of osteogenic markers, including Alp, Col I, Ocn and Runx2, was increased by HFC treatment. HFC induced the production of Osteocalcin, an important osteogenic-related protein, and activated the integrin-ERK signaling pathway. Conclusion HFC induces the osteogenic differentiation of hPDL cells potently, suggestive of an osteoinductive property. |
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