| 郑心怡,茅传圆,张翕,曾冠棋,李双,陆尔奕.白花丹素对TNF-α诱导的人牙周膜干细胞成骨分化的影响[J].口腔材料器械杂志,2017,26(3):118-123. |
| 白花丹素对TNF-α诱导的人牙周膜干细胞成骨分化的影响 |
| Effect of plumbagin on the osteogenic differentiation of TNF-α-induced human periodontal ligament stem cells |
| 投稿时间：2016-12-01 修订日期：2017-01-25 |
| DOI：10.11752/j.kqcl.2017.03.02 |
| 中文关键词: 白花丹素 肿瘤坏死因子-alpha 牙周膜干细胞 成骨分化 |
| 英文关键词:Plumbagin (PL) Tumor necrosis factor-alpha (TNF-α) Periodontal ligament stem cells (PDLSCs) Osteogenic differentiation |
| 基金项目: |
| 作者 | 单位 | E-mail | | 郑心怡 | 上海交通大学医学院附属第九人民医院口腔修复科·口腔医学院, 上海市口腔医学重点实验室, 上海 200011 | | | 茅传圆 | 上海交通大学医学院附属第九人民医院口腔修复科·口腔医学院, 上海市口腔医学重点实验室, 上海 200011 | | | 张翕 | 上海交通大学医学院附属第九人民医院口腔修复科·口腔医学院, 上海市口腔医学重点实验室, 上海 200011 | | | 曾冠棋 | 上海交通大学医学院附属第九人民医院口腔修复科·口腔医学院, 上海市口腔医学重点实验室, 上海 200011 | | | 李双 | 上海交通大学医学院附属第九人民医院口腔修复科·口腔医学院, 上海市口腔医学重点实验室, 上海 200011 | | | 陆尔奕 | 上海交通大学医学院附属第九人民医院口腔修复科·口腔医学院, 上海市口腔医学重点实验室, 上海 200011 | lueryi222@126.com |
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| 中文摘要: |
| 目的 研究白花丹素对TNF-α诱导的人牙周膜干细胞（periodontal ligament stem cells，PDLSCs）成骨分化的作用，以明确白花丹素的成骨机制及其作为牙周炎治疗药物的理论依据。方法 从健康的因正畸需要拔除的前磨牙中提取PDLSCs，实验分为4组：①对照组（Con）；②白花丹素组（PL）；③肿瘤坏死因子-α组（TNF-α）；④ TNF-α+PL组；采用CCK-8测试细胞活性，通过碱性磷酸酶（alkaline phosphatase，ALP）染色检测白花丹素对细胞的成骨分化趋势。最后，用Real-timePCR检测成骨表达标记物ALP、Runt相关转录因子2（Runt-related transcription factor 2，Runx2）及骨钙蛋白（osteocalcin，OCN）的mRNA表达量，以了解白花丹素对于PDLSCs的促成骨作用。结果 TNF-α组与TNF-α+PL组相对于CON组和PL组ALP、Runx2及OCN mRNA水平低，而TNF-α组与TNF-α+PL组间无显著差异，CON组和PL组间亦无显著性差异。结论 本研究证实了高浓度（10ng/ml）的TNF-α能够抑制PDLSCs的成骨分化，但白花丹素对TNF-α诱导的PDLSCs的成骨分化作用无显著效果。 |
| 英文摘要: |
| Objective In this study, we aimed to investigate the effect of plumbagin (PL) on the osteogenic differentiation of tumor necrosis factor (TNF)-α-induced human periodontal ligament stem cells (PDLSCs), so as to find out the potential of PL as a drug for chronic periodontitis. Methods PDLSCs were separated from healthy premolars extracted for the purpose of orthodontics. Samples were divided into 4 groups:① control group (Con); ② plumbagin group (PL); ③ TNF-α group (TNF-α) ④ TNF-α + PL group:CCK-8 was performed on PDLSCs viability to confirm the safety dosage of PL. Alkaline phosphatase (ALP) staining was used to detect the osteogenic differentiation trend of PL on PDLSCs. Finally, Real-time PCR analysis was performed to measure the mRNA level of osteogenic associated factors ALP, Runt-related transcription factor 2 (Runx2) and osteocalcin (OCN). Results The mRNA level of ALP, Runx2 and OCN in TNF-αand TNF-α+PL group is significantly lower than that of CON and PL group. However, no significant difference is observed between TNF-α and TNF-α+PL group, or between CON and PL group. Conclusion High concentration (10ng/ml) of TNF-α inhibits PDLSCs osteogenic differentiation, while PL was proven to have no obvious effects on the osteogenic differentiation of TNF-α-induced PDLSCs. |
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