| 黄永松,王婷婷,徐丽,芮艳.慢性阻塞性肺疾病中牙槽骨破坏与牙周膜VEGF-A165a表达水平的关系[J].口腔材料器械杂志,2022,31(1):28-33. |
| 慢性阻塞性肺疾病中牙槽骨破坏与牙周膜VEGF-A165a表达水平的关系 |
| The relationship between the alveolar bone destruction and the expression of VEGF-A165a in the periodontal ligament in chronic obstructive pulmonary disease |
| 投稿时间：2020-10-16 修订日期：2021-11-17 |
| DOI：10.11752/j.kqcl.2022.01.06 |
| 中文关键词: 慢性牙周炎 慢性阻塞性肺疾病 VEGF-A165a 牙槽骨破坏 |
| 英文关键词:Chronic periodontitis Chronic obstructive pulmonary disease VEGF-A165a Alveolar bone destruction |
| 基金项目:蚌埠医学院自然科学基金资助项目（编号：BYKY18105）；蚌埠医学院科技发展基金项目（编号：BYFY1891） |
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| 中文摘要: |
| 目的 探讨慢性阻塞性肺疾病中牙槽骨骨组织破坏与牙周膜VEGF-A165a表达水平的关系。方法 以11例单纯慢性牙周炎（CP）和12例慢性阻塞性肺疾病合并牙周患者（COPD+CP）为研究对象，分离培养2组拔牙病例的牙周膜成纤维细胞，制备条件培养基诱导破骨细胞（Trap+MNCs），提取RNA、逆转录及realtime PCR，检测病变牙周膜细胞中VEGF-A165a的相对表达量以及对应的破骨细胞诱导效率，并采用Perason相关性分析法评估两者的相关性。结果 COPD+CP组比CP组表达更高的VEGF-A165a水平，并可以诱导更多的破骨细胞形成（P<0.05）。重度和极重度COPD患者的牙周膜成纤维细胞中VEGF-A165a的相对表达量和破骨细胞诱导效率皆高于轻度COPD者。两组的VEGF-A165a的相对表达量与破骨细胞诱导效率成正相关性（P<0.05）。结论 COPD合并CP患者的牙周膜成纤维细胞中VEGF-A165a表达量和破骨细胞诱导效率均高于CP患者，该结果为临床干预COPD合并慢性牙周炎的治疗研究提供了一个潜在靶点。 |
| 英文摘要: |
| Objective The aim of this study was to investigate the role of VEGF-A165a in alveolar bone destruction in patients with chronic periodontitis (CP) and chronic obstructive pulmonary disease combined with periodontitis (COPD + CP). Methods Periodontal ligament fibroblasts were isolated and cultured from patients with COPD + CP and CP respectively. Conditioned medium was prepared to induce osteoclasts (Trap + MNCs). RNA extraction, reverse transcription and realtime PCR were performed to detect the relative expression level of VEGF-A 165a.The t-test and Perason correlation analysis were performed to evaluate the relationship between the expression level of VEGF-A165a and the efficiency of osteoclast induction in the two groups of diseases. Results COPD + CP group expressed higher levels of VEGF-A165a alternative splicing isoforms and induced more osteoclast formation (P<0.05). According to COPD pulmonary function classification, the expression levels of periodontal ligament fibroblasts, VEGF-A165a alternative splicing isoforms and osteoclast induction efficiency in patients with severe and very severe COPD were higher than those in the mild COPD group. The relative expression of VEGF-A165a was positively correlated with osteoclast induction efficiency in both groups. Conclusions The expression level of VEGF-A165a and the osteoclast induction efficiency were both higher in patients with chronic obstructive pulmonary disease than that in patients with chronic periodontitis, providing a potential target for clinical intervention in chronic obstructive pulmonary disease combined with periodontitis research. |
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