康文亭,周立伟,钟梅玲,朱勇军,李丽花,欧阳翔英.口腔胶原膜对两种骨再生相关细胞的作用[J].口腔材料器械杂志,2022,31(3):163-167,173.
口腔胶原膜对两种骨再生相关细胞的作用
Effects of oral collagen membrane on two kinds of bone regeneration related cells
投稿时间:2021-06-18  修订日期:2021-08-20
DOI:10.11752/j.kqcl.2022.03.03
中文关键词:  胶原膜  成纤维细胞  屏障作用  成骨细胞  黏附作用
英文关键词:Collagen membrane  Fibroblasts  Barrier function  Osteoblasts  Adhesion function
基金项目:国家重点研发计划政府间国际科技创新合作重点专项(编号:2018YFE0194300);深圳市发改委战略性新兴产业发展专项(编号:深发改[2019]561号)
作者单位E-mail
康文亭 深圳兰度生物材料有限公司, 广东省医用高分子植入材料工程技术研究中心, 深圳 518107  
周立伟 香港大学深圳医院口腔科, 深圳 518053  
钟梅玲 深圳兰度生物材料有限公司, 广东省医用高分子植入材料工程技术研究中心, 深圳 518107  
朱勇军 深圳兰度生物材料有限公司, 广东省医用高分子植入材料工程技术研究中心, 深圳 518107  
李丽花 深圳兰度生物材料有限公司, 广东省医用高分子植入材料工程技术研究中心, 深圳 518107  
欧阳翔英 北京大学口腔医院牙周科, 北京 100081 kqouyangxy@bjmu.edu.cn 
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中文摘要:
      目的 评价猪来源的脱细胞胶原膜在用于口腔引导骨再生时对成纤维细胞、成骨细胞的作用。方法 将成纤维细胞、成骨细胞分别接种在胶原膜表面,培养一定时间后通过扫描电镜和荧光共聚焦显微镜分别观察两种细胞在胶原膜上的生长迁移特点。结果 成纤维细胞与成骨细胞在膜表面均能良好附着,且生长状态良好。①将成纤维细胞接种在膜片的光滑面后,细胞没有向膜内迁移,仅沿膜表面生长;而接种在膜片的粗糙面后,细胞表现出向膜内部迁移的趋势。②将成骨细胞接种在膜片的粗糙面后,细胞黏附在胶原纤维上,形态舒展,状态良好。结论 胶原膜的光滑面能够对成纤维细胞发挥有效的屏障作用,同时成骨细胞能够在其粗糙面良好黏附。
英文摘要:
      Objective The aim of this study was to evaluate the effects of porcine-derived decellular collagen membrane on fibroblasts and osteoblasts to guide bone regeneration. Methods Fibroblasts and osteoblasts were respectively inoculated on the surface of the collagen membrane, and after cultured for a certain period of time, the growth and migration characteristics of the two kinds of cells were observed by scanning electron microscope and fluorescence confocal microscope. Results Both fibroblasts and osteoblasts could attach well to the membrane and grow well. ① After the fibroblasts were inoculated on the smooth surface of the membrane, the fibroblasts did not migrate into the interior of the membrane, but only grew along the surface. In contrast, while inoculated on the rough surface, the cells showed a tendency to migrate to the interior of the membrane. ② After the osteoblasts were seeded on the rough surface of the membrane, the osteoblasts adhered to the collagen fibers, showing elongated cell morphology. Conclusions The smooth surface of the collagen membrane can play an effective role as a barrier to fibroblasts. At the same time, osteoblasts can attach well to the rough surface of the membrane.
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