| 闫佳,曲贝贝,雷蕾.富血小板纤维蛋白膜对人牙龈成纤维细胞黏附生长的影响[J].口腔材料器械杂志,2026,35(2):85-88. |
| 富血小板纤维蛋白膜对人牙龈成纤维细胞黏附生长的影响 |
| Effects of platelet-rich fibrin membranes on the adhesion and growth of human gingival fibroblasts |
| 投稿时间：2024-03-10 修订日期：2025-12-30 |
| DOI：10.11752/j.kqcl.2026.02.04 |
| 中文关键词: 富血小板纤维蛋白膜 人牙龈成纤维细胞 细胞活力 碱性磷酸酶 黏附生长 |
| 英文关键词:Platelet-rich fibrin membrane Human gingival fibroblasts Cell viability Alkaline phosphatase Adhesion and growth |
| 基金项目:广东省自然科学基金项目(8151063201000065) |
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| 中文摘要: |
| 目的 研究富血小板纤维蛋白(platelet-rich fibrin,PRF)膜对人牙龈成纤维细胞(human gingivalfibroblasts,HGFs)黏附生长的影响。方法 组织块法分离培养HGFs，免疫组化鉴定来源；制备PRF膜、胶原膜；实验分为PRF膜组（PRF膜+HGFs）、胶原膜组（胶原膜+HGFs）、空白组（HGFs）。MTT法检测细胞活力；碱性磷酸酶试剂盒检测ALP活性；组织学和扫描电子显微镜观察细胞黏附生长情况。结果 MTT结果表明PRF膜有效增强HGFs活力（P<0.05）；第7 d和14 d，PRF膜组较胶原膜组、空白组的ALP活性明显增加（P<0.05）；组织学和扫描电镜观察，PRF膜组细胞均匀分布于膜表面和内部，数量多，大量伪足牢固黏附于PRF膜；胶原膜组细胞集中于膜表面和疏松层，数量少，伪足细小。结论 PRF膜能明显增强HGFs活力、促进HGFs分化，有利于HGFs的黏附生长。 |
| 英文摘要: |
| Objective To investigate the effects of platelet-rich fibrin (PRF) membranes on the adhesion and growth of human gingival fibroblasts (HGFs). Methods HGFs were isolated and cultured by the tissue explant method and identified by immunohistochemistry. PRF membranes and collagen membranes were prepared. The experiment included three groups: a PRF membrane group (PRF membrane + HGFs), a collagen membrane group (collagen membrane + HGFs), and a blank group (HGFs only). Cell viability was assessed by MTT assay, alkaline phosphatase (ALP) activity was measured using an ALP assay kit, and cell adhesion and growth were observed histologically and by scanning electron microscopy (SEM). Results MTT assay showed that PRF membranes enhanced HGF viability more effectively than collagen membranes (P<0.05). On days 7 and 14, ALP activity was significantly higher in the PRF membrane group than in the collagen membrane group and the blank group (P<0.05). Histological examination and SEM both showed that cells in the PRF membrane group were evenly distributed on the membrane surface and within the membrane, with greater cell numbers and numerous pseudopodia firmly attached to the PRF membrane, whereas in the collagen membrane group, cells were mainly distributed on the membrane surface and in the loose layer, with fewer cells and only small pseudopodia attached to the collagen membrane. Conclusion PRF membranes significantly enhanced HGF viability, promoted HGF differentiation, and facilitated the adhesion and growth of HGFs. |
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