曹轶婷,常雨丝,赖光云.不同根管冲洗液和冲洗方法对粪肠球菌清除作用的体外研究[J].口腔材料器械杂志,2020,29(2):16-21.
不同根管冲洗液和冲洗方法对粪肠球菌清除作用的体外研究
Efficiency of different rinsing fluids and irrigation techniques on reduction of Enterococcus faecalis in root canals in vitro
投稿时间：2019-09-15  修订日期：2019-12-09
DOI：10.11752/j.kqcl.2020.02.03
中文关键词:  根管冲洗  次氯酸钠  粪肠球菌  生物膜
英文关键词:Root canal irrigation  Sodium hypochlorite  Enterococcus faecalis  Biofilms
基金项目:上海市卫生健康委员会卫生行业临床研究专项（编号：20194Y0049），上海交通大学医学院附属第九人民医院种子基金资助（编号：JYZZ039）
作者单位E-mail
曹轶婷 上海交通大学医学院附属第九人民医院·口腔医学院儿童口腔科, 国家口腔疾病临床医学研究中心, 上海市口腔医学重点实验室, 上海市口腔医学研究所, 上海 200011  
常雨丝 上海交通大学医学院附属第九人民医院·口腔医学院儿童口腔科, 国家口腔疾病临床医学研究中心, 上海市口腔医学重点实验室, 上海市口腔医学研究所, 上海 200011  
赖光云 上海交通大学医学院附属第九人民医院·口腔医学院儿童口腔科, 国家口腔疾病临床医学研究中心, 上海市口腔医学重点实验室, 上海市口腔医学研究所, 上海 200011 lgy1985ivy@163.com 
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中文摘要:
      目的 比较不同冲洗液和冲洗方法对根管内粪肠球菌的清除效果。方法 建立120颗人完整单根管前磨牙粪肠球菌感染根管模型,随机分组,分别采用不同冲洗液(0.9% NaCl,0.5% NaClO,3% NaClO)及冲洗方法(注射针头冲洗,超声荡洗,RinsEndo系统处理,超声荡洗协同RinsEndo系统联用)进行根管清理。无菌吸潮纸尖取样,平板菌落计数法计算CFU值,计算细菌清除率,运用SPSS 22.0软件进行统计分析,P<0.05时差异具有统计学意义。结果 使用0.9% NaCl及0.5% NaClO冲洗液时,注射针头冲洗组细菌清除率明显低于超声荡洗及RinsEndo系统处理组(P<0.001);使用3% NaClO冲洗液的各组不同的冲洗方法,细菌清除率的差异无统计学意义(P=0.556)。而无论采用哪种冲洗方法,3% NaClO溶液的细菌清除效果均优于0.5% NaClO溶液和0.9% NaCl溶液(P<0.001)。结论 不同根管冲洗液在一定程度上会影响根管冲洗方法对根管内粪肠球菌的清除效果。
英文摘要:
      Objective The purpose of this study was to observe the bactericidal effect of different irrigation techniques and rinsing fluids on Enterococcus faecalis in root canals. Methods One hundred and twenty intact human single canal premolar teeth were selected to build models of Enterococcus faecalis infected root canals. All the teeth were randomly and averagely divided into 12 groups. Bacteria samples of root canals were collected by using sterile paper point and then were cultured onto culture dishes. In the end, the CFUs were counted, and the clearance of Enterococcus faecalis was calculated to evaluate the efficiency of different irrigation techniques on the reduction of Enterococcus faecalis in root canals. The software SPSS 22.0 was applied for the statistical analysis. There was significant difference when P<0.05. Results When 0.9% NaCl and 0.5% NaClO was used as the rinsing fluid, the clearance of Enterococcus faecalis in the group using Syrnge needle has much lower than that in groups using Ultrasonic Irrigation and RinsEndo (P<0.001). There was no significant difference among all groups using 3% NaClO as rinsing fluids (P=0.556). Among different irrigation techniques, the clearance of Enterococcus faecalis in the group using 3% NaClO as rinsing fluids was higher than that in groups using either 0.5% NaClO or 0.9% NaCl (P<0.001). Conclusion The working efficiency of irrigation techniques on the removal of Enterococcus faecalis in the root canal could be affected by the rinsing fluid to a certain extent.
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